MSC Serum free medium is specially optimized for the culture and expansion of human mesenchymal stem cells (hMSCs), under completely serum-free and xeno-free conditions. MSC Medium supports the long-term cell culture and expansion of MSCs for multiple passages, while maintaining their various differentiation.
Serum-free, xeno-free
High harvest，up to106-107cells from 15cm umbilical cord within 14 days
High expansion foldHigh expansion fold，6-10 fold increase in 72 hours, stable passaging for 20 generations, uniform cell morphology
Ready to use, no coating required, retains multilineage differentiation
potential (trilineage differentiation) and CD markers
The MSC Serum-free Medium MH01 is a serum-free, xeno-free medium specifically optimized for the isolation and expansion of human mesenchymal stem cells (hMSCs) under fully serum-free, phenol red-free, and xeno-free conditions.
The MSC Serum-free Medium MH02 require 2% serum substitute supplementation.Other content is the same as MH01.
Contains no animal-derived components, recombinant trypsin, with clear composition, suitable for use in stem cell research. MH01 and MH02 are better used with RF01.
1000L Fully-automatic Liquid Filling line
Stainless steel liquid blending system
Equipped with 200L & 1000L fully-automatic liquid filling line
B+A grade filling workshop
Stainless steel liquid dosing system to support CIP & SIP
GMP certified facility
| Product Name | Specifications | Constitute | Explanation |
|---|---|---|---|
| MSC Serum-free Medium MH01 | 1 Kit | Basal Medium （500 mL/1 L）+Supplement | Xeno-free,Liquid forms, Phenol red-free |
| MSC Serum-free Medium MH02 | 1 Kit | Basal Medium （500 mL/1 L）+Supplement | Liquid forms, Phenol red-free.An additional 2% serum replacement is required |
| OptiVitro Recombinant Trypsin Digestive Solution RF01 | 200 mL | —— | No animal-derived components, recombinant protein, Meets pharmacopoeia standards |
A.FBS containing；B.Medium Ⅰ（Y）;C.Medium Ⅲ（I）;D.MSC SFM
Image captured at 10X magnification.
| Product Name | Specifications | Constitute | Explanation |
|---|---|---|---|
| MSC Serum-free Medium MH01 | 1 Kit | Basal Medium （500 mL/1 L）+Supplement | Xeno-free,Liquid forms, Phenol red-free |
| MSC Serum-free Medium MH02 | 1 Kit | Basal Medium （500 mL/1 L）+Supplement | Liquid forms, Phenol red-free.An additional 2% serum replacement is required |
| OptiVitro Recombinant Trypsin Digestive Solution RF01 | 200 mL | —— | No animal-derived components, recombinant protein, Meets pharmacopoeia standards |
Test Conditions: Cells: UC-MSCs at passages 6-8； Seeding Density: 8,000 cells/cm²； Passage Duration: 72 h； Digestion Reagent: Excell Recombinant Trypsin RF01 (RF000-N031)
MH02, combined with common serum substitutes, effectively maintains MSC growth, demonstrates superior proliferation rate vs. conventional serum-based media (MEM-α + 5% serum substitute), maintains normal cell diameter range, and reduces serum substitute costs.
| Medium | MH02+ 2% serum substitute | ME01 | MEM-α+5% HELIOS | ||
|---|---|---|---|---|---|
| STEMCELL | HELIOS | PL BioScience | |||
| CD73 | 100% | 99.9% | 100% | 99.8% | 99.8% |
| CD90 | 100% | 99.9% | 99.9% | 99.9% | 100% |
| CD105 | 99.8% | 99.6% | 99.5% | 99.5% | 99.9% |
| CD45，CD34，CD14，CD19，HLA-DR | <1% | <0.8% | <0.4% | <0.4% | <0.1% |
MH02 maintains MSC phenotype across serum substitutes, with flow cytometry positivity rates:
CD73/CD90/CD105: >99%
CD45/CD34/CD14/CD19/HLA-DR:<1%
MH02 enables higher exosome yield and purity
| Seeding Density（T175) | Cell Harvest | Supernatant Volume | Exo-Concentration（particles/ml） | Avg. Exosome Yield（particles/ml/cell） | Purity（particles/ug） | |
|---|---|---|---|---|---|---|
| Excell | 8000cells/cm² | 7.06E6 | 20 mL | 3.87E10 +/- 4.79E8 | 5.48E3 | 1.36E9 |
| Sa* | 8000cells/cm² | 7.12E6 | 20 mL | 1.99E10 +/- 3.08E8 | 2.79E3 | 0.66E9 |
| EXO 30-150nm | ||
|---|---|---|
| Excell | Concentration average（particles/mL） | 9.1E9 |
| Percentage（%） | 23.567 | |
| Sa* | Concentration average（particles/mL） | 5.49E9 |
| Percentage（%） | 27.598 |
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