ExCell Bio has built an advanced culture media factory with large-scale, informationization and internationalization. We can provide international-leading high-performance and high-quality classical cell culture Fetal bovin serum. We can provide different types of FBS for different cell lines .
Cell culture
Cell culture involves growing cells in vitro under controlled conditions (e.g., temperature, pH, nutrients) to study behavior, develop therapies, or produce biologics. Key steps include isolating cells from tissues (primary culture) or using immortalized cell lines (e.g., HeLa), maintaining sterility, and subculturing via enzymatic detachment (e.g., trypsin). Applications span drug testing, cancer research, and biomanufacturing. Challenges include contamination risks (bacteria, mycoplasma) and genetic instability in cell lines. Advances like defined media and antibiotics revolutionized the field.
Downstream experiment
Downstream experiments in biology focus on validating and analyzing data post-initial discovery, such as confirming gene/protein functions or mechanistic insights. Key techniques include molecular analyses (e.g., qRT-PCR, Western blot), functional studies (e.g., gene knockout, CRISPR), and interaction assays (e.g., co-IP, yeast two-hybrid). These experiments bridge lab findings to applications like drug development or biomanufacturing, addressing challenges like contamination control. For example, flow cell biofilm assays assess microbial interactions, while electrophysiology explores neuronal circuits.
Cell cryopreservation
Cryopreservation is vital for the long-term storage and preservation of cell products. Cells are harvested and resuspended in a cryopreservation medium that protects them from freezing damage. They are then aliquoted into cryogenic vials and subjected to a controlled freezing process to prevent the formation of ice crystals that could damage the cells. The vials are stored in liquid nitrogen, which maintains a temperature of around -196°C, ensuring the cells remain viable for extended periods. This process allows for the banking of cells, facilitating their use in future treatments and research.
