Product Classification
| Catalog No. | Product Name | Specification |
| MH000-N011 | MSC Serum-free Medium MH01 | 500ml |
| MH000-N012 | MSC Serum-free Medium MH01 | 1000ml |
Product Overview
The MSC Serum-free Medium MH01 is a serum-free, xeno-free medium specifically optimized for the isolation and expansion of human mesenchymal stem cells (hMSCs) under fully serum-free, phenol red-free, and xeno-free conditions. It supports primary MSC isolation using explant and enzymatic digestion methods and enables expansion over multiple passages, preserving the stability of cell surface markers.
● Serum-free, xeno-free.
● High primary yield: 10⁶–10⁷ cells from a 15 cm umbilical cord in 14 days.
● High expansion: uniform morphology, 6–10x fold expansion in 72 hours, stable for 20 passages.
● Ready-to-use: retains multilineage differentiation potential and CD markers.
● Independently developed, stable supply, GMP.
Specifications & Data
Fig1. A statistical graph of the MSC expansion fold during passage culture, tested over 20 continuous passages using MH01 and a competitor product. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours. |
Fig2. A statistical graph of the MSC doubling time during passage culture, tested over 20 continuous passages using MH01 and a competitor product. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours. |
Fig3. A statistical graph showing the change in cell diameter of MSCs during passage culture, tested over 20 continuous passages using MH01 and a competitor product. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours. |
Fig 4. Representative images of MSCs during continuous 12-passage culture, tested using MH01. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours. |
Fig 5.Representative flow cytometry images of MSCs at passage 5 (P5), tested using MH01. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours. |
Fig 6. Immunosuppressive ability test of MSCs at passage 8 (P8), using MH01. The comparison was made between culture media containing 5% FBS and 5% hPL. |
Documents
Manual_MHN01X
MSDS_MHN01X
Application
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