ExCellBio 293 Serum-Free media

Empowering Scalable Biopharmaceutical Production

In the fast-growing field of protein expression cell and gene therapy, 293 Serum-Free Medium has become an indispensable solution for high-performance suspension cell culture. Engineered to eliminate reliance on animal-derived components, this advanced medium ensures consistent cell expansion, enhances viral vector yields, and meets stringent regulatory requirements—critical for modern therapeutic development.

Animal origin-free, chemically-defined formulationSustains high viability in transfected suspension 293 cellsSupports high-density growth of multiple 293 cell linesEnables efficient transfection and high-level protein expressionGMP-grade

PRODUCT

OptiVitro® 293 Serum-free Medium TransExp HE02

OptiVitro® 293 Serum-free Medium TransExp HE02 significantly enhances protein production in transient transfections.

GMP-grade

Chemically defined and animal-derived components free

Buy HE02

OptiVitro® 293 Serum-free Medium VirTrans HE03

Support for the rapid expansion, high-density growth, and plasmid transfection of adherent HEK 293-derived cells (such as 293T, 293F, etc.)

GMP-grade

Enables high-titer LV and AAV production

Buy HE03

OptiVitro® 293 Serum-free Feed Medium HA02/HA03

A chemically-defined, animal-derived components free medium specifically developed to support the growth and transfection of multiple suspension 293 cell lines.

GMP-grade

Only feed once, easy to operate

Buy CE01/CE02/CE03

High-performance 293 serum-free medium

Well-defined chemical composition, facilitating high-expression production

	Basal Media	Feed Media
Protein expression media	OptiVitro® 293 Serum-free Medium TransExp HE02	OptiVitro® 293 Serum-free Feed Medium HA02
Virus package media	OptiVitro® 293 Serum-free Medium TransExp HE03	OptiVitro® 293 Serum-free Feed Medium HA03

OptiVitro® 293 Transient Expression Medium

OptiVitro® 293 Serum-free Medium TransExp HE02/HA02 chemically-defined medium without any animal-derived components or protein, specifically designed for protein production. It is suitable for high-density culture and transient transfection protein expression of HEK293-derived suspension cell lines, especially Expi293 cells.

Product Name	Cat.#	Specification
OptiVitro® 293 Serum-free Medium TransExp HE02	HE000-N051	1L, liquid
	HE000-N052	1L, powder
	HE000-N062	10L, powder
	HE000-N063	100L, powder
OptiVitro® 293 Serum-free Feed Medium TransExp HA02	HA000-N011	100mL, liquid
	HA000-N012	1L, liquid
	HA000-N021	1L Powder
	HA000-N022	10L Powder
OptiVitro® Glucose solution	M101382C	100mL, liquid

UE000-N092

Product Features

diams;No animal-derived components, chemically defined
diams;Supports single-cloning of various CHO and 293 cell types
&diams;Significantly improves single-clone proliferation rate
&diams;Perfectly replaces cloning media containing hydrolysates

OptiVitro® 293 Serum-free Culture Medium, Empowering Cells with Excellent Productivity

Protein Expression Medium

Basal Media	Feed Media
OptiVitro® 293 Serum-free Medium TransExp HE02	OptiVitro® 293 Serum-free Feed Medium HA02

Virus Packaging Medium

Basal Media	Feed Media
OptiVitro® 293 Serum-free Medium TransExp HE03	OptiVitro® 293 Serum-free Feed Medium HA02
OptiVitro® 293 Serum-free Medium TransExp HE03	OptiVitro® 293 Serum-free Feed Medium HA03

♦Serum-free, no animal-derived components, chemically defined
♦Maintains high-density cell growth under optimized conditions
♦User-friendly workflow - Streamlined protocols minimize hands-on time and operational complexity
♦High-yield performance - Delivers over 1g/L IgG expression levels (compatible with feed supplementation for further productivity boost) Clone Product

♦Serum-free, no animal-derived components, chemically defined
♦Supports rapid expansion and high-density growth of various suspension 293 cells
♦Enables high-efficiency transfection and virus production; with feed addition, it significantly enhances virus packaging yield
♦Fully independently developed, stable supply, GMP-grade, supporting clinical application and commercialization Clone Product

Resources and Support

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Media Formulation Development and Customization

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