Jan. 20, 2026

How to Choose Serum-free Freezing Medium for Stem Cells

Selecting the right Serum-free freezing medium is crucial for the successful preservation of stem cells. The choice of medium can significantly impact cell viability and overall recovery after thawing. With multiple options available, understanding the key features of Serum-free Media and selecting one that aligns with specific research goals is essential.

How to Choose Serum-free Freezing Medium for Stem Cells 

Understanding Serum-free Freezing Media

 

Serum-free freezing mediums are designed to support the survival of cells during cryopreservation without the use of animal-derived components. These media provide a chemically defined environment that minimizes variability in experimental results. When choosing a Serum-free freezing medium, researchers should consider formulations that offer support for specific cell types, such as CHO-K1 and CHO-S cells. The ability to maintain high cell viability post-thaw is crucial for downstream applications.

 

Key Features to Consider

 

When evaluating Serum-free freezing media, look for animal-origin free formulations. The incorporation of chemically defined components ensures reproducibility and consistency in experiments. The OptiVitro® CHO Serum-free Freed Medium TransExp CA06 exemplifies an ideal choice, as it is specifically developed for high-density cultures and does not require additional glutamine. Products that include a single glucose feed can streamline freezing protocols and improve post-thaw protein production, which is of utmost importance in biopharmaceutical applications.

 

Making the Right Choice

 

Opting for a Serum-free freezing medium that meets the specific requirements of your cell type can enhance the success of cryopreservation efforts. The right medium not only optimizes cell viability but also provides a more consistent platform for future experimental work.

 

ExCell Bio stands out in this niche by offering high-quality Serum-free Media, such as the OptiVitro® CHO Serum-free Basal Medium. Their commitment to providing reliable, chemically defined formulations ensures researchers can focus on their scientific objectives without the added variability associated with serum-based systems.


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